Biotechnologist

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  1030 Wien, Landstraße
 12.07.2018

Kurzvorstellung

Ich bin ein Biotechnologe der sich für nachhaltige Produktionsverfharen von Bioprodukten, z.b Biofactories, Nutrazeutika, pflanzlicher Arzneimittel, Umweltsanierung interessiert. Ich freue mich auf das in F&E Arbeiten so wie in der Kommunikation.

Auszug Referenzen (5)

"Herr Dr. A. [...] war bei uns für die Optimierung einer Error- Prone Methode für einen Milchsäure produzierenden MO mit sehr viel Einsatz tätig."
Researcher - Molecular biology
Dr. Markus Kroutil
Tätigkeitszeitraum

3/2017 – 8/2017

Tätigkeitsbeschreibung

- I elaborated methods for genetic improvement of B. coagulans strains
- Performed HTS (high through-put screening) and HPLC analysis
- Carried out literature, database mining and NGS data analysis
- Wrote reports and SOPs
- Trained coworkers

Eingesetzte Qualifikationen

Biotechnologie, Naturwissenschaft


"Dr. [...] was a visiting researcher in my group at the Plant Breeding and Genetics Laboratory at the IAEA Laboratories."
Visiting Scientist
Bradley John Till
Tätigkeitszeitraum

10/2016 – 12/2016

Tätigkeitsbeschreibung

- Optimization of pre-NGS amplicon fragmentation protocols
- Writing a scientific paper
- Coworker supervision

Eingesetzte Qualifikationen

Naturwissenschaft


"I was an independent researcher, learning and training myself in different fields for future projects."
Independent Scientist
Andrea Tramontano
Tätigkeitszeitraum

1/2015 – 4/2016

Tätigkeitsbeschreibung

I extended my knowledge in several fields including:
Next Generation Sequencing, Induced genome mutagenesis, Genome editing, Exome capture. I trained myself in:
NGS data analysis, at Medical University of Vienna
languages: German, acheived B2, completed B2/C1 course, at VHS Vienna. I also:

Eingesetzte Qualifikationen

Biotechnologie, Angewandte Forschung


"[...] Tramontan hat am AIT ein Jahr lang als Postdoc in einem Projekt über Markerentwicklung bei Kartoffeln mitgearbeitet"
Freelance scientist
Kundenname anonymisiert
Tätigkeitszeitraum

4/2013 – 3/2014

Tätigkeitsbeschreibung

Based on NGS deep sequencing data I established a screening method for large sample populations and MAS (marker assisted selection) for viral and fungal resistance NBS-LRR genes in S. tuberosum. I conducted literature research, carried out all molecular biology steps prior to next generation sequencing, analyzed data, designed allele-specific primers, conducted wet-lab trials, contributed to building genetic map, gave presentations, supervised students.
I also took part in another NGS based project aiming at identifying natural variants of industrial interest of the S. tuberosum GBSS (granule-bound starch synthase) enzyme.

Eingesetzte Qualifikationen

Medizintechnik / Labortechnik, Biotechnologie, Interkulturelle Kommunikation, Forschung & Entwicklung (allg.), Angewandte Forschung, Biologie, Aus- / Weiterbildung


"Dr. [...] [...] hat seine Doktorarbeit in meiner Gruppe am Max Planck Institut für Pflanzenzüchtungsforschung in Köln durchgeführt."
Staff Scientist
Prof. Andreas Bachmair
Tätigkeitszeitraum

7/2006 – 4/2011

Tätigkeitsbeschreibung

Between Cologne and Vienna I carried out my PhD research and a bridging postdoc.

I was responsible for the literature research and for laboratory research.
I developed methods for gene mutagenesis crop plants. I gave presentations, wrote scientific publications, participated in international congresses, organized scientific events and supervised students.

Eingesetzte Qualifikationen

Event Marketing / Event management, Angewandte Forschung, Kommunikationswissenschaft, Biologie


Ich biete

Forschung, Wissenschaft, Bildung
  • Angewandte Forschung
    7 Jahre, 5 Monate Erfahrung
  • Biologie
    5 Jahre, 10 Monate Erfahrung
Technik, Ingenieurwesen
  • Biotechnologie
    3 Jahre, 1 Monat Erfahrung
  • Biochemie

Projekt‐ & Berufserfahrung

Researcher - Molecular biology
Junbunzlauer Austria AG, Pernhofen
3/2017 – 8/2017 (6 Monate)
Life Sciences
Tätigkeitszeitraum

3/2017 – 8/2017

Tätigkeitsbeschreibung

- I elaborated methods for genetic improvement of B. coagulans strains
- Performed HTS (high through-put screening) and HPLC analysis
- Carried out literature, database mining and NGS data analysis
- Wrote reports and SOPs
- Trained coworkers

Eingesetzte Qualifikationen

Biotechnologie, Naturwissenschaft


Visiting Scientist
IAEA, Seibersdorf
10/2016 – 12/2016 (3 Monate)
Life Sciences
Tätigkeitszeitraum

10/2016 – 12/2016

Tätigkeitsbeschreibung

- Optimization of pre-NGS amplicon fragmentation protocols
- Writing a scientific paper
- Coworker supervision

Eingesetzte Qualifikationen

Naturwissenschaft


MOLECULAR BIOLOGIST
BOKU Vienna, Department of Plant Biotechnology/IAE, Wien
6/2016 – 8/2016 (3 Monate)
Life Sciences
Tätigkeitszeitraum

6/2016 – 8/2016

Tätigkeitsbeschreibung

- RNA-seq of the multipurpose plant Jatropha curcas (physic nut).
- TILLING by Sequencing of J. curcas to identify genes for oil biosynthesis, toxicity and abiotic stress resistance.
- Student training

Eingesetzte Qualifikationen

Biotechnologie, Angewandte Forschung, Naturwissenschaft, Aus- / Weiterbildung


Independent Scientist
Kundenname anonymisiert, Wien
1/2015 – 4/2016 (1 Jahr, 4 Monate)
Life Sciences
Tätigkeitszeitraum

1/2015 – 4/2016

Tätigkeitsbeschreibung

I extended my knowledge in several fields including:
Next Generation Sequencing, Induced genome mutagenesis, Genome editing, Exome capture. I trained myself in:
NGS data analysis, at Medical University of Vienna
languages: German, acheived B2, completed B2/C1 course, at VHS Vienna. I also:

Eingesetzte Qualifikationen

Biotechnologie, Angewandte Forschung


Freelance scientist
AIT (Austrian Institute of Technologie), Tulln
4/2013 – 3/2014 (1 Jahr)
Life Sciences
Tätigkeitszeitraum

4/2013 – 3/2014

Tätigkeitsbeschreibung

Based on NGS deep sequencing data I established a screening method for large sample populations and MAS (marker assisted selection) for viral and fungal resistance NBS-LRR genes in S. tuberosum. I conducted literature research, carried out all molecular biology steps prior to next generation sequencing, analyzed data, designed allele-specific primers, conducted wet-lab trials, contributed to building genetic map, gave presentations, supervised students.
I also took part in another NGS based project aiming at identifying natural variants of industrial interest of the S. tuberosum GBSS (granule-bound starch synthase) enzyme.

Eingesetzte Qualifikationen

Medizintechnik / Labortechnik, Biotechnologie, Interkulturelle Kommunikation, Forschung & Entwicklung (allg.), Angewandte Forschung, Biologie, Aus- / Weiterbildung


Staff Scientist
MPIPZ (Köln) - MFPL (Wien), Köln und Wien
7/2006 – 4/2011 (4 Jahre, 10 Monate)
Life Sciences
Tätigkeitszeitraum

7/2006 – 4/2011

Tätigkeitsbeschreibung

Between Cologne and Vienna I carried out my PhD research and a bridging postdoc.

I was responsible for the literature research and for laboratory research.
I developed methods for gene mutagenesis crop plants. I gave presentations, wrote scientific publications, participated in international congresses, organized scientific events and supervised students.

Eingesetzte Qualifikationen

Event Marketing / Event management, Angewandte Forschung, Kommunikationswissenschaft, Biologie


Zertifikate

Team Management in Science (by Leadershisp Sculptor®/EMBO)
November 2012

“Project Management” (by PENTALOG®)
September 2011

Ausbildung

Naturwissenschaften - Genetik
(PhD)
Jahr: 2011
Ort: Universität zu Köln

Pflanzen Biotechnologie
(Master Naturwissenschaften)
Jahr: 2005
Ort: Università di Napoli "Federico II"

Qualifikationen

HARD SKILLS

MOLECULAR BIOLOGY
PCR, RT PCR, Inverse PCR, Multiplex PCR, AS-PCR, Error-prone PCR, qPCR, molecular markers: SNPs, CAPs, SSR, ISSR, RAPDs.

BIOCHEMISTRY
DNA / RNA work Cloning, Agarose Gel Electrophoresis, Southern / Western Blotting, Protein isolation, Protein heterologous expression, Antibody purification, SDS-PAGE, Flow-cytometry, ELISA,, HPLC (Chromeleon), HTS (High Throughput Screening).

MICROBIOLOGY Genetic manipulation of E. coli, A. tumefaciens, Bacillus coagulans. “Heat-shock” transformation, electroporation.

PLANT METHODS
In vitro and in vivo manipulation of T. aestivum, A. thaliana, H. vulgare, S. tuberosum, J. curcas.

NEXT GENERATION SEQUENCING
Illumina, Ion Torrent, workflow management, Data Analysis, NGS Quality Control, Amplicon Sequencing, RNA-seq

GENOMICS
SNP discovery, NBS-profiling, MAS linkage mapping in tetraploid species (first experience), gene annotation, Induced mutagenesis, TILLING by Sequencing, Eco-TILLING

COMPUTER SKILLS
MS Office, Open Office, BioEdit, Sequencer, IGV (Integrated Genome Viewer), Bio-Linux, Clone Manager, CLC Main Workbench, Primer3, CODDLE, QuantityOne (Biorad), Genome Databases, BLAST, MSA (Multi Sequence Alignment), Tetraploid Map, Image J, HTS (High Throughput Screening), Microlab STAR (Hamilton), PROSize, Covaris, Chromeleon, NGS Pipelines, CODDLE, Provean, SIFT, Adobe Acrobat, Adobe Photoshop, SAP.

HARDWARE
All Thermocyclers (PCR), Fragment Analyzer (Applied Biosystems), Hamilton Liquid Handling Workstation, HPLC, Covaris Sonicator, Nanodrop, Qubit, Optical Microscopy.


SOFT SKILLS

GxP

COMMUNICATION
Communication skills gained through professional experience, further training and free time activities
“Talented in presentations”
“Able to simplify complex concepts”
Client relations

TEAM WORKING
Ability and joy to work in a multicultural team / environment
Ability to perform with autonomy, as well as part of a team

ORGANIZATION / MANAGEMENT
Management and coordination of multidisciplinary projects
Ability to establish collaborations with partners in different fields

HOBBIES
Gastronomy (cooking, baking), arts (music, theatre), sports (volleyball, jogging, swimming)

LANGUAGE SKILLS
Italian: Native Speaker
English: Fluent
German: Fluent
Spanish: Fluent
Serbo/Croatian: beginner

Über mich

I am an open person, who enjoyes working in contact with people. I am excited about getting to know new cultures through my work, therefore I would also like a job that allows me to travel.
I would like to work in applied research and possibly bridging between different institutions. For me not only the laboratory research is important, but the real transformation of the results into products.

Persönliche Daten

Sprache
  • Italienisch (Muttersprache)
  • Englisch (Fließend)
  • Deutsch (Fließend)
  • Spanisch (Fließend)
  • Serbisch (Grundkenntnisse)
Reisebereitschaft
Weltweit
Arbeitserlaubnis
  • Europäische Union
Profilaufrufe
717
Alter
37
Berufserfahrung
12 Jahre und 2 Monate (seit 07/2006)
Projektleitung
1 Jahr

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