QA Technician

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95246 Klaipeda
24.11.2018

Kurzvorstellung

I have 3+ laboratory work experience. I performed microbiological assays, DNA handling techniques, protein expression and isolation and immunoassay tests. I also performed routine laboratory duties: maintenance of equipment, infrastructure and tools.

Ich biete

Technik, Ingenieurwesen
  • Biotechnologie
  • Biochemie
Forschung, Wissenschaft, Bildung
  • Biologie
Management, Unternehmen, Strategie
  • Qualitätssicherungssysteme

Projekt‐ & Berufserfahrung

QA Technician (Festanstellung)
Philip Morris International, Klaipėda
5/2018 – offen (8 Monate)
Konsumgüterindustrie
Tätigkeitszeitraum

5/2018 – offen

Tätigkeitsbeschreibung

Quality Assurance Technician

Eingesetzte Qualifikationen

Qualitätssicherungssysteme


Verwalterin (Festanstellung)
"Baltic Medics", Klaipėda
11/2017 – 4/2018 (6 Monate)
Gesundheitswesen
Tätigkeitszeitraum

11/2017 – 4/2018

Tätigkeitsbeschreibung

I was responsible for the registration of patients‘ specimens, reporting the results via e-mail or by phone, communication with clients and patients, organizing the referral letters given by the doctors as well as the hard copies of the results, which were later sent to the ordering physician. I also placed orders for required supplies and organized specimen deliveries to our subcontractors.

Eingesetzte Qualifikationen

Archivierung, Auftragsverwaltung, Büroorganisation, Call Center, Datenpflege / -erfassung, Dokumentenmanagement, Kundenberatung


Intern
Vilnius University, Vilnius
2/2013 – 5/2013 (4 Monate)
Hochschulen und Forschungseinrichtungen
Tätigkeitszeitraum

2/2013 – 5/2013

Tätigkeitsbeschreibung

Prepared selected yeast strains for microscopy
Applied indirect immunofluorescence microscopy for cell visualization

Eingesetzte Qualifikationen

Biotechnologie, Biochemie, Biologie


Technician
Nature Research Centre, Vilnius
1/2013 – 7/2014 (1 Jahr, 7 Monate)
Hochschulen und Forschungseinrichtungen
Tätigkeitszeitraum

1/2013 – 7/2014

Tätigkeitsbeschreibung

Prepared growth media for budding yeast
Concentrated yeast K2 toxin
Cultivated budding yeast cells
Measured optical density
Carried out toxin-cell binding assays
Tested the K2 toxin activity in the presence of different polysaccharides
Determined the binding of the toxin to yeast mutants with altered levels of cell wall receptors

Eingesetzte Qualifikationen

Biotechnologie, Biochemie, Biologie


Biologist (Festanstellung)
Nature Research Centre, Vilnius
11/2012 – 7/2014 (1 Jahr, 9 Monate)
Hochschulen und Forschungseinrichtungen
Tätigkeitszeitraum

11/2012 – 7/2014

Tätigkeitsbeschreibung

Prepared required chemical solutions for experiments
Provided general support to laboratory specialists in the completion of routine and experimental laboratory procedures
Cultivated cells
Measured optical density
Constructed a recombinant plasmid bearing the budding yeast toxin encoding gene
Constructed E. coli transformants containing the recombinant plasmid
Adjusted the growth conditions of the bacterial transformants
Developed a protocol for production and purification of the recombinant protein
Using indirect ELISA and Western blot determined the sensitivity and specificity to antibodies against the recombinant protein
Prepared growth media for bacteria and yeast
Guided junior students, supported their experiments
Constructed vectors bearing tagged yeast genes (tagged proteins used for localisation and isolation)
Curated the laboratory’s budding yeast and bacteria strain collection
Carried out microbiological assays
Carried out maintenance of laboratory supply inventory and reported shortages to management
Conducted maintenance of laboratory tools and equipment
Reported repairs or replacements to supervisor
Visualized the results and wrote reports later used in publications or conferences

Eingesetzte Qualifikationen

Biotechnologie, Biochemie, Biologie


Technician
Nature Research Centre, Vilnius
1/2012 – 12/2012 (1 Jahr)
Hochschulen und Forschungseinrichtungen
Tätigkeitszeitraum

1/2012 – 12/2012

Tätigkeitsbeschreibung

Prepared growth media for budding yeast
Concentrated yeast K2 toxin
Cultivated budding yeast cells
Measured optical density
Carried out toxin-cell binding assays
Determined the binding of the toxin to yeast mutants with altered levels of cell wall receptors

Eingesetzte Qualifikationen

Biotechnologie, Biochemie, Biologie


Intern
Synlab, Vilnius
3/2010 – 4/2010 (2 Monate)
Gesundheitswesen
Tätigkeitszeitraum

3/2010 – 4/2010

Tätigkeitsbeschreibung

Learned about the diagnostic methods conducted in the laboratory
Sorted and labelled samples accurately
Recorded the results
Performed general clinical test (rapid urine tests, blood type tests, enzyme immunoassay tests, allergen-specific assays)
Performed routine molecular biology tests (PCR, DNA gel electrophoresis) for detection of sexually transmitted diseases

Eingesetzte Qualifikationen

Biotechnologie, Biochemie, Biologie


Ausbildung

Biochemistry
(Master of Science)
Jahr: 2013
Ort: Vilnius, Lithuania

Bioengineering
(Bachelor of Science)
Jahr: 2011
Ort: Vilnius, Lithuania

Qualifikationen

Microorganism cultivation and isolation
Molecular cloning
Bacterial transformations (chemical transformation and electroporation)
Yeast transformations
Cell cloning
Cryopreservation of cultured cells
Disruption of cells by sonication, manual grinding (with mortar and pestle) of frozen cells with liquid nitrogen, treating cells with glass beads
Protein isolation and detection using SDS-PAGE electrophoresis
Indirect ELISA
Western Blot
DNA detection and purification using DNA electrophoresis
PCR
Cell analysis using indirect immunofluorescence microscopy
Curating the laboratory’s strain collection

Use and calibration of measuring devices (pH-meter, milligram balance…)
Preparation of growth media for microorganisms
Chemical solution preparation
Sterilization of tools, glassware, supplies using an autoclave
Working in clean zones provided by vertical laminar flow work station and UV cabinet
Recording research procedures and results
Keeping working stations clean and washing glassware on regular basis

Über mich

I am currently looking for a full time position in a life science company. I have 3+ years laboratory work experience. I have been an intern in a medical diagnostics laboratory, worked as a technician and a biologist in a scientific institution. I performed microbiological sample analysis, molecular biology techniques, protein expression and isolation, and immunoassays. I also conducted routine laboratory duties: maintenance of equipment, infrastructure and tools, prepared required growth media and chemical solutions.

Persönliche Daten

Sprache
  • Litauisch (Muttersprache)
  • Englisch (Fließend)
  • Deutsch (Gut)
  • Russisch (Gut)
Reisebereitschaft
Weltweit
Arbeitserlaubnis
  • Europäische Union
  • Schweiz
Profilaufrufe
445
Alter
30
Berufserfahrung
8 Jahre und 9 Monate (seit 03/2010)

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