freiberufler PharmD, PhD (Toxicology) auf

PharmD, PhD (Toxicology)

  • auf Anfrage
  • 70193 Stuttgart
  • Weltweit
  • pt  |  en  |  de
  • 15.11.2017


Toxicologie, Pharmacologie, Biologie, Stammzellbiologie, Zellbiologie, Zellkulturforschung, Molekularbiologie
Erweiterte Kenntnisse:
Biomonitoring, Pharmakovigilanz, Biotechnologie


  • Biochemie
  • Biologie
  • Biotechnologie
  • Pharmaforschung
  • Pharmazie

Projekt‐ & Berufserfahrung

Universidade de Lisboa, Faculty of Pharmacy, 1649-003
1/2013 – 6/2017 (4 Jahre, 6 Monate)

1/2013 – 6/2017


Applying 3D cell culture models for long-term hepatic in vitro toxicology applications.
I developed 3D models for differentiated cells (#1, #2) and stem cells (3). This gave me a strong experimental experience in cell and tissue engineering, histology, and gene expression as well as in implementing functional assays for the evaluation of cells biotransformation capacity and toxicological response (#1, #2). I had also the opportunity to work with HPLC methodologies for drug metabolites quantification and for the thiolomic profile determination (4, 5). In parallel with my project, I participated in several other projects comprehending: the study of stem cell conditioned medium for wound healing and tissue regeneration (3); for the modulation of breast cancer migration and invasion; the study of nevirapine toxicity mechanisms using a dynamic 3D culture of rat primary hepatocytes (5); and the study of efavirenz short- and long-term neurotoxic mechanisms in a rat model (4).

Publications from doctoral thesis (% referes to my contribution to each project)

# 1. [...], M., Correia, J. C., Camões, S. P., Oliveira, N. G., Cruz, P., Cruz, H., Castro, M., Ruas, J. L., Santos, J. M., Miranda, J. P. (2017) The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells. Arch Toxicol. 91(6):2469-89. (70% - study design, experimental work, data analysis and manuscript writing)
# 2. [...], M.#, Freyer, N., Knöspel, F., Oliveira, N. G., Barcia, R., Cruz, P. E., Cruz, H., Castro, M., Santos, J. M., Zeilinger, K., Miranda, J. P. (2017) Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro. Arch Toxicol. 91(4):1815-32. (80% - study design, experimental work, data analysis and manuscript writing)
3. Santos, J. M., Camões, S. P., Filipe, E., [...], M., Barcia, R. N., Filipe, M., Teixeira, M., Simoes, S., Gaspar, M., Mosqueira, D., Nascimento, D. S., Pinto-Do, O. P., Cruz, P., Cruz, H., Castro, M., Miranda, J. P. (2015) Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing. Stem Cell Res Ther. 6:90. (30% - experimental work, data analysis and manuscript writing)

4. Grilo, N. M., Joao Correia, M., Miranda, J. P., [...], M., Serpa, J., Matilde Marques, M., Monteiro, E. C., Antunes, A. M. M., Diogo, L. N., Pereira, S. A. (2017) Unmasking efavirenz neurotoxicity: Time matters to the underlying mechanisms. Eur J Pharm Sci. 105:47-54. (20% - experimental work, data analysis and manuscript writing)
5. Pinheiro, P. F., Pereira, S. A., Harjivan, S. G., Martins, I. L., Marinho, A. T., [...], M., Jacob, C. C., Oliveira, N. G., Castro, M. F., Marques, M. M., Antunes, A. M., Miranda, J. P. (2017) Hepatocyte spheroids as a competent in vitro system for drug biotransformation studies: nevirapine as a bioactivation case study. Arch Toxicol. 91(3):1199-211. (15% - experimental work and data analysis)
6. Fernandes, A. S., Florido, A., Saraiva, N., Cerqueira, S., Ramalhete, S., [...], M., Cabral, M. F., Miranda, J. P., Castro, M., Costa, J., Oliveira, N. G. (2015) Role of the Copper(II) Complex Cu[15]pyN5 in Intracellular ROS and Breast Cancer Cell Motility and Invasion. Chem Biol Drug Des. 86(4):578-88. (15% - experimental work and data analysis)
7. Fernandes, A. S., Costa, J., Gaspar, J., Rueff, J., Cabral, M. F., [...], M., Castro, M., Oliveira, N. G. (2012) Development of pyridine-containing macrocyclic copper(II) complexes: potential role in the redox modulation of oxaliplatin toxicity in human breast cells. Free Radic Res. 46(9):1157-66. (10% - experimental work and data analysis)
8. Gonçalves, S., Fernandes, A. S., Oliveira, N. G., Marques, J., Costa, J., Cabral, M. F., Miranda, J., [...], M., Guerreiro, P. S., Castro, M. (2012) Cytotoxic effects of cadmium in mammary epithelial cells: protective role of the macrocycle [15]pyN5. Food Chem Toxicol. 50(6):2180-7. (5% - experimental work and data analysis)

Eingesetzte Qualifikationen

Biotechnologie, Pharmaforschung, Pharmakologie, Pharmazie, Biologie


PhD in Pharmacy from Universidade de Lisboa, Faculty of Pharmacy
Lissabon, Portugal
Pharmaceutical Sciences
Lissabon, Portugal
Community Pharmacy
Lissabon, Portugal
Clinical Pharmacy
Hospital São Francisco Xavier, Centro Hospitalar d

Persönliche Daten

  • Deutsch (Gut)
  • Englisch (Fließend)
  • Portugiesisch (Muttersprache)
  • Europäische Union
11 Jahre und 3 Monate (seit 01/2013)


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